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Efficient transformation of a Yersinia enterocolitica strain (serotype O:3) by pBR322 DNA |
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| Authors: | Luciano Rodríguez-Solórzano JoséC Rodríguez JoséM Ortiz Antolín Mellado |
| Institution: | Department of Microbiology, Faculty of Medicine, University of Santander, Poligono de Cazonña, Santander, Spain;Department of Biochemistry, Faculty of Medicine, University of Santander, Poligono de Cazoña, Santander, Spain |
| Abstract: | Abstract The Yersinia enterocolitica strain MS201 (serotype O:3) was transformed by pBR322 DNA extracted from an Escherichia coli strain. The pBR322 DNA extracted from an Escherichia coli strain. The pBR322 DNA extracted from the transformed Y. enterocolitica is able to transform plasmid-free MS201 at a significantly higher frequency, suggesting the existence of a restriction-modification system in MS201. |
| Keywords: | Transformation Yersinia enterocolitica pBR322 restriction-modification system |