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Efficient Haploid Induction in Microspore Suspension Culture of Aesculus hippocastanum and Karyotype Analysis
Authors:D. Ćalić  S. Zdravković-Korać  S. Jevremović  M. Guć-Šćekić  Lj. Radojević
Affiliation:(1) Department of Plant Physiology, Institute for Biological Research, “Siniša Stanković”, 29 Novembar 142, 11000 Belgrade;(2) Department of Genetics, Institute for Mother and Child Health Care, Radoja Dakića 8, 11000 Belgrade
Abstract:Suspension culture was more efficient method for haploid production than anther culture. All analysed androgenic regenerants originating from embryogenic microspores in suspension culture of Aesculus hippocastanum L. had a haploid number of chromosomes (n=20), while 50 % of those derived from anther culture were diploids. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:androgenesis  anther culture  horse chestnut
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