Optimization of <Emphasis Type="Italic">Verticillium lecanii</Emphasis> spore production in solid-state fermentation on sugarcane bagasse |
| |
Authors: | Yujie Shi Xiangqun Xu Yang Zhu |
| |
Institution: | (1) School of Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China;(2) TNO Quality of Life, Business Unit Bioscience, P. O. Box 360, 3700 AJ Zeist, Netherlands |
| |
Abstract: | Verticillium lecanii is an entomopathogen with high potential in biological control of pests. We developed a solid-state fermentation with sugarcane
bagasse as carrier absorbing liquid medium to propagate V. lecanii spores. Using statistical experimental design, we optimized the medium composition for spore production. We first used one-factor-at-a-time
design to identify corn flour and yeast extract as the best carbon and nitrogen sources for the spore production of V. lecanii. Then, we used two-level fractional factorial design to confirm corn flour, yeast extract, and KH2PO4 as important factors significantly affecting V. lecanii spore production. Finally, we optimized these selected variables using a central composite design and response surface method.
The optimal medium composition was (grams per liter): corn flour 35.79, yeast 8.69, KH2PO4 1.63, K2HPO4 0.325, and MgSO4 0.325. Under optimal conditions, spore production reached 1.1 × 1010 spores/g dried carrier, much higher than that on wheat bran (1.7 × 109 spores/g initial dry matter). |
| |
Keywords: | Verticillium lecanii Spore production Central composite design Solid-state fermentation Biological control Response surface methodology |
本文献已被 SpringerLink 等数据库收录! |
|