Design of multidye systems for FRET-based applications

A new solid phase approach, based on orthogonal protective group strategy utilizing Fmoc and DMTr groups, was used to assemble linear polymeric chains with pending groups at desired locations. A compound synthesized using four different fluorophores with consequently overlapping absorption and emission spectra (pyrene, perylene, fluorescein and TAMRA) was shown to fluoresce at 570 nm when excited at 330 nm, demonstrating sequential energy transfer across four chromophores.