Mechanical purification of torpedo stage somatic embryos of Daucus carota L. |
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| Authors: | David L. Rodriguez Sherry L. Kitto Ken M. Lomax |
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| Affiliation: | (1) Delaware Agricultural Experiment Station, Department of Plant and Soil Sciences, College of Agricultural Sciences, University of Delaware, 19717-1303 Newark, DE, USA;(2) Department of Agricultural Engineering, College of Agricultural Sciences, University of Delaware, 19717-1303 Newark, DE, USA;(3) Present address: Agricultural Research Division, American Cyanamid Company, 08540 Princeton, NJ, USA |
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| Abstract: | A filtering unit was designed and constructed that concentrates and purifies torpedo-stage embryos, from carrot (Daucus carota L.) embryo suspensions. The filtering unit was composed of three sections, each containing a screen disk. Purification efficiency of a series of three screen size combinations was evaluated using 10 to 15 ml of 23- to 30-day-old embryo suspensions. Under optimal conditions where the first screen was a 1000 micron in size, >84% of the total number of torpedo embryos were concentrated in the purified fraction. Purified fractions contained more than double the percentage of torpedo embryos when compared to unfiltered suspensions. This filtering unit provided a simple method for concentrating and purifying torpedo embryos from a suspension. |
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| Keywords: | Daucus carota L. embryo purification mechanical filtration somatic embryos |
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