| 一种高效获取基因5′末端的RACE方法 |
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| 引用本文： | 罗聪,何新华,陈虎,韦泳丽,李明娟.一种高效获取基因5′末端的RACE方法[J].植物生理学通讯,2011(4):409-414. |
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| 作者姓名： | 罗聪 何新华 陈虎 韦泳丽 李明娟 |
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| 作者单位： | 广西大学农学院;广西作物遗传改良与生物技术重点实验室; |
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| 基金项目： | 广西高等学校优秀人才资助计划项目(桂教人201065); 广西大学博士后启动基金(20090042); 广西自然科学基金(2011GXNSFA018115); 广西教育厅研究生创新计划项目(105931001011) |
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| 摘 要： | RACE技术是一种快速高效克隆基因5′末端和3′末端的方法,是获取基因全长的主要手段之一,但是RACE技术本身也存在一些缺点。我们在前人改良的RACE技术基础上进一步优化RACE技术,获得了一种操作简单、快速、高效、成本低廉的改良RACE方法,该方法适合于大量基因5′末端的获取,可以在普通实验室推广应用。
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| 关 键 词： | RACE TdT 方法改良 |
A High-Efficient Method of RACE Technique for Obtaining the Gene 5' End |
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| LUO Cong,HE Xin-Hua,CHEN Hu,WEI Yong-Li,LI Ming-Juan College of Agriculture,Guangxi University,Nanning ,China, Guangxi Crop Genetic Improvement , Biotechnology Lab,Nanning.A High-Efficient Method of RACE Technique for Obtaining the Gene 5' End[J].Plant Physiology Communications,2011(4):409-414. |
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| Authors: | LUO Cong HE Xin-Hua CHEN Hu WEI Yong-Li LI Ming-Juan College of Agriculture Guangxi University Nanning China Guangxi Crop Genetic Improvement Biotechnology Lab Nanning |
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| Institution: | LUO Cong~1,HE Xin-Hua~(1,2,*),CHEN Hu~1,WEI Yong-Li~1,LI Ming-Juan~1 1 College of Agriculture,Guangxi University,Nanning 530004,China,2 Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007 |
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| Abstract: | RACE technique is a rapid and effective method for cloning gene 5' end and 3' end,and one of main methods to obtain full-length genes.However,RACE technique has its own shortcomings.We further improved the previous RACE protocols and obtained a modified RACE technique which is simple,rapid,high-efficient and low-cost.Our modified RACE technique is suitable for obtaining large number of genes 5' end and can be used in ordinary laboratories. |
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| Keywords: | RACE TdT modified method |
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