| 原子力显微镜研究UV-B对I型胶原结构的影响 |
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| 引用本文： | 梁志红,周长忍,蔡基业,王小燕. 原子力显微镜研究UV-B对I型胶原结构的影响[J]. 激光生物学报, 2004, 13(3): 207-209 |
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| 作者姓名： | 梁志红 周长忍 蔡基业 王小燕 |
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| 作者单位： | 暨南大学实验技术中心,中国广东,广州,510632;暨南大学生命和技术学院,中国广东,广州,510632;青岛大学,中国山东,青岛,266003 |
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| 基金项目： | 国家自然科学基金(60278014),广东省科技攻关(2002C32404)。 |
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| 摘 要： | 利用原子力显微镜(AFM)成像技术观察胶原蛋白溶液在UV-B照射前后形态的变化,发现UV-B引起胶原纤维交联度的增加,当交联达一定程度后,照射时间的增加对交联度增加的影响不明显。AFM作为一种高分辨的表面分析仪器,为分子生物学领域的研究提供了一种新的手段。是探讨胶原光作用机理直观、有效的方法。
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| 关 键 词： | 原子力显微镜(AFM) I型胶原 UV-B 交联 |
| 文章编号： | 1007-7146(2004)03-0207-03 |
| 收稿时间： | 2004-03-05 |
| 修稿时间： | 2004-03-05 |
Effects of UV-B on the Morphological of I Type Collagen Fibril by AFM |
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| LIANG Zhi-hong,ZHOU Chang-ren,CAI Ji-ye,WANG Xiao-yan. Effects of UV-B on the Morphological of I Type Collagen Fibril by AFM[J]. ACTA Laser Biology Sinica, 2004, 13(3): 207-209 |
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| Authors: | LIANG Zhi-hong ZHOU Chang-ren CAI Ji-ye WANG Xiao-yan |
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| Affiliation: | LIANG Zhi-hong~1,ZHOU Chang-ren~2,CAI Ji-ye~2,WANG Xiao-yan~3 |
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| Abstract: | I type collagen from bovine skin radiated under UV-B were imaged by Atomic force microscope (AFM). It was found an obvious increase in the degree of collagen crosslinking after UV-B radiating. The degree of crosslinking didn't have further change after 3 hours exposure. The crossinking behavior of collagen fibril under UV-B exposure can be clearly observed by means of AFM. It is proved that AFM is an excellent technique for the study of collagen fibril. |
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| Keywords: | atomic force microscope (AFM) I type collagen UV-B crosslinking |
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