Immunohistochemical localization and quantitative analysis of cellular glutathione peroxidase in foetal and neonatal rat tissues: Fluorescence microscopy image analysis |
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Authors: | Kohtaro Asayama Kazushige Dobashi Yasusuke Kawada Takaya Nakane Akira Kawaoi and Shinpei Nakazawa |
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Institution: | (1) Departments Pediatrics, Yamanashi Medical University, 1110 Shimakato. Tamahocho, 409-38 Yamanashi, Japan;(2) Departments Pathology, Yamanashi Medical University, 1110 Shimakato. Tamahocho, 409-38 Yamanashi, Japan |
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Abstract: | Summary To quantitate the developmental changes in selenium-dependent cellular glutathione peroxidase during the perinatal period,
tissue sections from foetal (day 12 to day 22) and neonatal (day 6) rats were stained immunohistochemically using specific
polyclonal antiserum. The intensity of the staining was quantified by fluorescence microscopy image analysis. There was a
general trend of enriched glutathione peroxidase in the epithelial linings and metabolically active sites. Significant fluorescence
was detected in cardiomyocytes, hepatocytes, renal tubular epithelium, bronchiolar epithelium and intestinal epithelium at
day 15. The intensity increased in a stepwise manner therafter. The overall increase in the intensity of staining in the heart,
liver, kidneys, lungs and intestine was 1.5-, 2.3-, 1.6-, 1.7- and 3.0-fold, respectively. The phase of most rapid increase
occurred during the foetal period in the liver, intestine and heart. In the kidneys and lungs, glutathione peroxidase increased
significantly during foetal life, and to a similar extent postnatally. These results suggest that the intracellular H2O2-scavenging system develops during the foetal period as an essential mechanism for living under atmospheric oxygen conditions.
The late development observed in the kidneys and lungs is consistent with the relative biological immaturity of these organs
in full-term neonates. |
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