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FAB CID-MS/MS characterization of tetrasaccharide tri- and tetrasulfate derived from the antigenic determinant recognized by the anti-chondroitin sulfate monoclonal antibody MO-225 |
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| Authors: | Tadashi II Masayuki Kubota Takashi Hirano Mamoru Ohashi Keiichi Yoshida and Sakaru Suzuki |
| Institution: | (1) Department of Applied Physics and Chemistry, The University of Electro-Communications, 182 Chofu, Tokyo, Japan;(2) Tokyo Research Institute, Seikagaku Corporation, Higashiyamato-shi, 189 Tokyo, Japan;(3) Institute for Molecular Science of Medicine, Aichi Medical University, 480-11 Nagakute, Aichi, Japan |
| Abstract: | The fast atom bombardment (FAB) collision induced dissociation (CID)-mass spectrometry/mass spectrometry (MS/MS) technique was successfully applied to characterize and identify the structures of the immunoreactive trisulfated and tetrasulfated tetrasaccharides that were obtained from the chondroitin sulfate in a shark fin using a treatment with chondroitinase ABC.Abbreviations FABMS
fast atom bombardment mass spectrometry
- CID
collision induced dissociation
- MS/MS
mass spectrometry/mass spectrometry
-  UA2S-GalNAc6S
2-acetamido-2-deoxy-3-O-(2-O-sulfo- -d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-galactose
- UA-GalNAc4S
2-acetamido-2-deoxy-3-O-(-d-gluco-4-enepyranosyluronic acid)-4-O-sulfo-d-galactose
- UA-GalNAcDiS
2-acetamido-2-deoxy-3-O-(-d-gluco-4-enepyranosyluronic acid)-4,6-di-O-sulfo-d-galactose |
| Keywords: | FABMS CID MS/MS trisulfated tetrasaccharide tetrasulfated tetrasaccharide chondroitin sulfate shark fin monoclonal antibody MO-225 glycosaminoglycan |
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