Comparative evaluation of pectolytic and proteolytic enzyme production by free and immobilized cells of some strains of the phytopathogenic Erwinia chrysanthemi |
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Authors: | N YO Muyima M Zamxaka N T Mazomba |
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Institution: | (1) Environmental and Natural Products Biotechnology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, P/B X1314, Alice 5700, South Africa, ZM |
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Abstract: | Whole cells of the phytopathogenic Erwinia chrysanthemi strains were immobilized in k-carrageenan and grown in high-calcium Xanthomonas campestris medium containing sodium polypectate as carbon source. All the strains used survived immobilization into k-carrageenan beads.
Immobilized E. chrysanthemi strains displayed higher pectolytic and proteolytic enzyme activities than free cells in liquid suspension. Carrageenan immobilization
techniques could provide a system to mimic the conditions of E. chrysanthemi cells in the infected plant tissue. This could prompt a thorough study of the factors governing the biosynthesis of virulence
factors by this bacterium. Journal of Industrial Microbiology & Biotechnology (2001) 27, 215–219.
Received 04 April 2001/ Accepted in revised form 12 June 2001 |
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Keywords: | : immobilized Erwinia chrysanthemi cells pectolytic enzyme proteolytic enzyme |
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