An efficient and versatile approach for the construction of oligonucleotide microarrays |
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| Authors: | Mahajan S Kumar P Gupta K C |
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| Institution: | Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi-110,007, India. |
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| Abstract: | A new immobilization chemistry for covalent attachment of phosphorylated oligonucleotides on epoxy-activated glass surface via opening of oxirane ring is described. The proposed strategy results in excellent immobilization efficiency, spot homogeneity, and morphology. The constructed microarray was successfully demonstrated for discrimination of nucleotide mismatches. |
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