凝固剂对水稻花药培养愈伤组织诱导的影响

本文初步研究了不同类型的凝固剂对水稻花药培养愈伤组织形成的影响。结果发现，用Gelrite、马铃薯淀粉、甘薯淀粉，可溶性淀粉代替琼脂可明显促进水稻花药培养愈伤组织的产生而尤以5.0％马铃薯淀粉为最佳。出愈率比琼脂增加5.2倍，达液体培养水平。以8个不同基因型，为材料研究发现，5．0％马铃薯淀粉作凝固剂，有7个材料出愈率高于对照，最高的BCl63比对照增加7.75倍，平均增加1.15倍。另外，以5.0％马铃薯淀粉作凝固剂代替0.8％琼脂可降低成本30％。因此，用马铃薯淀粉作凝固剂在水稻花药培养中可能具有潜在的应用价值。

The Effect of Gelling Agents on Callus Induction in Anther Culture of Rice

The present study was designed to examine the effect of different kinds of gelling agents on the frequency of callus induction in anther culture of rice. Approximately 5 times the amount of Calli were produced in liquid as compared to an agar solidified medium. A significantly higher percentage of calli also was observed by the use of gelrite, potato starch, sweet potato starch and sulable starch instead of agar as the gelling agents. Potato starch was the best one among the tested gelling agents. The percentage of calli on the potato starch medium was 5.2 times higher than that on agar medium and was similar then that of the liquid medium. Eight genotypes were used in the study, seven genotypes showed higher percentage of callus induction in potato starch modium and the average frequency was 1.15 times higher than the control. The highest frequency was found in variety BC 163 and it was 7.75 times higher as compared to the agar medium. Besides, potato starch was cheaper than agar when used in the medium, Results showed that agar in anther culture medium could be replaced by potato starch.