Mitochondrial acyl carrier proteins in <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> are predominantly soluble matrix proteins and none can be confirmed as subunits of respiratory Complex I |
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Authors: | Etienne H Meyer Joshua L Heazlewood A Harvey Millar |
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Institution: | (1) ARC Center of Excellence in Plant Energy Biology, University of Western Australia, 4th Floor MCS Building M316, 35 Stirling Highway, 6009 Crawley, WA, Australia |
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Abstract: | Arabidopsis mitochondria are predicted to contain three acyl carrier proteins (ACPs). These small proteins are involved in fatty acid
and lipoic acid synthesis in other organisms and have been previously reported to be subunits of respiratory Complex I in
mitochondria in mammals, fungi and plants. Recently, the mammalian mitochondrial ACP (mtACP) has been shown to be largely
a soluble matrix protein but also to be minimally associated with Complex I (Cronan et al. 2005), consistent with its involvement in synthesis of lipoic acid for TCA cycle decarboxylating dehydrogenases in the matrix
but contrary to earlier claims it was primarily a Complex I subunit. We have investigated the localization of the ACPs in
Arabidopsis mitochondria. Evidence is presented that mtACP1 and mtACP2 dominate the ACP composition in Arabidopsis mitochondria, and both are present in the mitochondrial matrix rather than in the membrane. No significant amounts of mtACPs
were detected in Complex I isolated by blue native gel electrophoresis, rather mtACPs were detected at low molecular mass
in the soluble fraction, showing that in A. thaliana mtACPs are predominately free soluble matrix proteins. |
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Keywords: | Acyl carrier proteins Mitochondria Complex I Arabidopsis |
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