| 肽核酸相关技术在杂交检测中的应用 |
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| 引用本文： | 张晨,杨瑞馥. 肽核酸相关技术在杂交检测中的应用[J]. 生物技术通讯, 2000, 11(3): 226-230 |
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| 作者姓名： | 张晨 杨瑞馥 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 摘 要： | 肽核酸是一种寡核苷酸的类似物，它是由丹麦哥本哈根大学的Ｎｉｅｌｓｅｎ、Ｅｇｈｏｌｍ等人首先发明合成的。肽核酸与传统的寡核苷酸相比，骨架结构发生了根要变化。肽核酸的电中性骨架有许多ＤＮＡ所不具备的性质，例舅高灵敏度、高特异性、非盐依赖性等，从而使它成为一种优良的寡核苷酸的取代物，尤其是杂交检测领域。
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| 关 键 词： | 肽核酸 寡核苷酸 杂交 检测 PNA |
Application of peptide nucleic acid associated techniques in hybridization detection |
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| Zhang Chen,Yang Ruifu. Application of peptide nucleic acid associated techniques in hybridization detection[J]. Letters in Biotechnology, 2000, 11(3): 226-230 |
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| Authors: | Zhang Chen Yang Ruifu |
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| Abstract: | People has developed some kinds of mimics of oligonucleotides , among them there is Peptide Nucleic Acid(PNA), which was first synthesized by scientists at the University of Copenhagen(Nielsen, Egholm et al).The idea of PNA means a drastic change in the backbone of nucleic acid. A neutral backbone imparts PNA with many excellent characteristics in its application in the fields of molecular biology, especially as hybridization probes. |
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| Keywords: | peptide nucleic acid oligonucleotide hybridization detection |
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