梓醇对氧糖剥夺诱导PC1 2 细胞凋亡的保护作用

目的：观察梓醇对氧糖剥夺（OGD）诱导PC12细胞凋亡的保护作用。方法：采用Hoechst 33258 DNA染色法，四甲基偶氮唑盐（MTT）检测细胞活性；化学比色法测定乳酸脱氢酶（LDH）的释放量，用流式细胞技术检测细胞凋亡比例以及P53和Bcl-2蛋白。结果：OGD可导致PC12细胞活力明显下降，LDH释放量增加、P53蛋白表达上升，Bcl-2蛋白表达下降。梓醇可明显改善细胞形态结构，显著降低LDH释放量、降低P53蛋白的表达，提高Bcl-2蛋白的表达，降低细胞凋亡率。结论：梓醇通过调节细胞凋亡相关基因的表达而抑制细胞凋亡。

Catalpol Protects Rats' Pheochromocytoma Cells Against Oxygen-glucose Deprivation-induced Injury

Objective: To investigate the effect of catalpol in apoptosis in PC12 cells induced by oxygen -glucose deprivation (OGD). Methods: The morphological changes were observed by Hoechst 33258 staining assay; the cell viability was analyzed by MTT; the apoptosis rate, the protein of P53 and Bcl-2 were measured by flow cytometry analysis (FCM). Results: OGD could result in the decrease of cell viability, the increase of release of LDH, the dropping of Bcl-2 protein expression of PC12 cell, the rising of P53 protein expression of PC12 cell. Catalpol could improve the morphology of cells. It could increase bcl-2 protein expression, decrease P53 protein expression and the release of LDH, and reduce apoptotic rate. Conclusion: Catalpol could inhibit PC12 cells apoptosis by regulating related genes of apoptosis.