Optimization of fermentation parameters for the biomass and DHA production of Schizochytrium limacinum OUC88 using response surface methodology |
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| Institution: | 1. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, No. 30 Puzhu South Road, Nanjing 211816, People''s Republic of China;2. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing 211816, People''s Republic of China;3. School of Pharmacy, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing 211816, People''s Republic of China;4. Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening and Co-innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, People''s Republic of China |
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| Abstract: | Fermentation parameters for biomass and DHA production of Schizochytrium limacinum OUC88 in a fermenter (working volume 7 L) were optimized using Plackett–Burman and central composite rotatable design. Out of 10 factors studied by Plackett–Burman design, 4 influenced the biomass production significantly. Central composite rotatable design was used to optimize the significant factors and response surface plots were generated. Using these response surface plots and point prediction, optimized values of the factors were determined as follows temperature (°C) 23 °C, aeration rate 1.48 L min?1 L?1, agitation 250 rpm and inoculum cells in mid-exponential phase, the maximum yield of DCW and DHA were 24.1 and 4.7 g L?1, respectively. These predicted values were also verified by validation experiments. |
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