Antioxidant response of a novel Streptomyces sp. M3004 isolated from legume rhizosphere to H2O2 and paraquat |
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| Institution: | 1. Ondokuz May?s University, Science and Arts Faculty, Department of Biology, 55139 Kurupelit, Samsun, Turkey;2. Dokuz Eylül University, Education Faculty, Department of Chemistry, 35150 Buca, ?zmir, Turkey;1. Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India;2. Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan;3. Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093-0687, USA;3. From the Department of Biochemistry and Molecular Biology, the Centre for Blood Research and;4. Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada and;5. Centre for Molecular and Structural Biochemistry, School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom;1. State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China;2. Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China;1. Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia;2. National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, UK;4. UM Omics Centre, University of Malaya, Kuala Lumpur, Malaysia;5. Division of Microbiology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia |
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| Abstract: | This paper aims to investigate the effect of H2O2 and paraquat on the activities of superoxide dismutase (SOD) and catalase (CAT), and membrane lipid peroxidation (LPO) levels in newly isolated Streptomyces sp. M3004. SOD activities of Streptomyces sp. M3004, grown in 10 mM and 30 mM H2O2, were significantly lower than the control cultures. On the other hand, as an antioxidant enzyme, CAT activity in both H2O2 treatment conditions increased significantly compared with the control. These activity values in 10 mM and 30 mM H2O2 treatment on the 48th hour of incubation were 3.8- and 6.6-fold higher than the control, respectively. SOD activity decreased significantly with respect to paraquat concentration, which was added at the start of the incubation. CAT activities increased significantly in 1.0 mM and 3.0 mM paraquat treatments compared to control. As an indicative marker of membrane damage, LPO levels of the novel isolate Streptomyces sp. M3004 treated with H2O2, and paraquat stress conditions were significantly higher than the control. Nevertheless, compared with the 30 mM H2O2 in both treatment conditions, LPO levels in 10 mM H2O2 were significantly higher. The decreases in SOD activities in paraquat and H2O2 treatment conditions resulted in the increases in the LPO levels although it increases in CAT activities. |
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