Effect of soybean oil and Tween 80 on the production of botryosphaeran by Botryosphaeria rhodina MAMB-05 |
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| Institution: | 1. Departo. de Bioquímica e Biotecnologia-CCE, Universidade Estadual de Londrina, CEP 86051-990, Londrina, PR, Brazil;2. Departo. de Ciência e Tecnologia de Alimentos-CCA, Universidade Estadual de Londrina, CEP 86051-990, Londrina, PR, Brazil;3. Departo. de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente, SP, Brazil;1. Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China;2. Amoy – BUCT Industrial Bio-technovation Institute, Amoy 361022, PR China;3. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China;1. Research Institute of Surface Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China;2. School of Aeronautics, Northwestern Polytechnical University, Xi’an 710072, PR China;3. School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an 710032, PR China;1. CCET, Universidade de Caxias do Sul, Caxias do Sul 95070-560, Brazil;2. Departamento de Física, PUC-Rio, Rio de Janeiro 22453-900, Brazil;3. IF, Universidade Federal do Rio Grande do Sul, Porto Alegre 91509-970, Brazil;4. IFGW-DFA, Universidade Estadual de Campinas, Campinas 13083-970, Brazil;5. Plasmar Tecnologia Ltda., Caxias do Sul 95030-775, Brazil;1. Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China;2. School of Life Sciences, Shandong University, Jinan 250100, PR China |
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| Abstract: | The addition of soybean oil and Tween 80 was evaluated with the objective of increasing the production of botryosphaeran, an exopolysaccharide (EPS) of the (1 → 3;1 → 6)-β-d-glucan type produced by the fungus Botryosphaeria rhodina MAMB-05. Factorial design and analysis by response surface methodology was developed to select the main factors that would affect and enhance EPS production. The optimized culture conditions were: 40 g l?1 glucose with 10 ml l?1 soybean oil, and 4.5 ml l?1 Tween 80, during 72 h cultivation at 28 °C (180 rpm) and initial pH 5.7. The predicted result for botryosphaeran production was 8.22 ± 1.36 g l?1, and compared with the experimental value of 7.74 ± 0.13 g l?1. Partial characterization of the botryosphaeran produced under the optimized conditions showed one type of polysaccharide with β-glycosidic linkages containing glucose as monosaccharide. |
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