| H2S介导ABA诱导蚕豆气孔运动的生理机制研究 |
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| 引用本文： | 刘菁,侯智慧,赵方贵,刘新. H2S介导ABA诱导蚕豆气孔运动的生理机制研究[J]. 西北植物学报, 2011, 31(2): 298-304 |
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| 作者姓名： | 刘菁 侯智慧 赵方贵 刘新 |
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| 作者单位： | 青岛农业大学,生命科学学院,山东青岛,266109 |
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| 基金项目： | 国家自然科学基金，山东省自然科学基金，植物生理学与生物化学国家重点实验室开放课题 |
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| 摘 要： | 以蚕豆为实验材料,利用药理学实验和分光光度法,研究了ABA处理及ABA与H2S合成抑制剂共处理对蚕豆气孔运动的影响,以及体内H2S水平、H2S合成酶L-/D-半胱氨酸脱巯基酶(磷酸吡哆盐依赖性酶)活性变化.结果表明:(1)光下H2S的合成抑制剂羧甲氧基胺半盐酸盐(AOA)、羟氨(NH2OH)、L-/D-半胱氨酸脱巯基酶分解产物C3H3KO3+NH3均明显抑制ABA诱导的蚕豆气孔关闭;(2)外源ABA能够明显提高叶片的H2S水平及L-/D-半胱氨酸脱巯基酶活性;(3)AOA、NH2OH、C3H3KO3和NH3均可以逆转ABA所引起的H2S水平及L-/D-半胱氨酸脱巯基酶活性的升高.研究发现,ABA可通过增强L-/D-半胱氨酸脱巯基酶活性,促进L-/D-半胱氨酸分解生成H2S,进而诱导蚕豆气孔关闭.
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| 关 键 词： | 蚕豆 硫化氢 脱落酸 气孔运动 |
Hydrogen Sulfide Mediates ABA-induced Stomatal Closure of Vicia faba L. |
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| LIU Jing,HOU Zhi-hui,ZHAO Fang-gui,LIU Xin. Hydrogen Sulfide Mediates ABA-induced Stomatal Closure of Vicia faba L.[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(2): 298-304 |
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| Authors: | LIU Jing HOU Zhi-hui ZHAO Fang-gui LIU Xin |
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| Affiliation: | (College of Life Sciences,Qingdao Agricultural University,Qingdao,Shandong 266109,China) |
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| Abstract: | Through pharmacological combined with spectrophotography to study the effects of ABA and ABA with inhibitors of H2S synthesis on stomatal closure of Vicia faba L.,H2S generation and L-/D-cysteine desulfhydrase(pyridoxalphosphate dependent enzymes) activity.The results showed that AOA and NH2OH,inhibitors of pyridoxal-5′-phosphate-dependent enzymes,C3H3KO3+NH3,products of L-/D-cysteine desulfhydrase all inhibited ABA-induced stomatal closure of Vicia faba L.obviously under light condition.Treatment with ABA induced H2S generation and increased L-/D-cysteine desulfhydrase activity.AOA,NH2OH,C3H3KO3+NH3 reduced H2S generation induced by ABA and the activity increase of L-/D-cysteine desulfhydrase.Those results suggested that ABA enhanced the activity of L-/D-cysteine desulfhydrase,inducing the accumulation of H2S via L-/D-cysteine resolving,and finally induced stomatal closure of Vicia faba L.. |
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| Keywords: | Vicia faba L. hydrogen sulfide abscisic acid stomatal movement |
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