| 红豆杉内生真菌次生代谢物的分离鉴定及其抗菌活性 分析 |
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| 引用本文： | 陈淑娟,刘佳佳,龚汉祥,杨栋梁.红豆杉内生真菌次生代谢物的分离鉴定及其抗菌活性 分析[J].生物工程学报,2009,25(3):368-374. |
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| 作者姓名： | 陈淑娟 刘佳佳 龚汉祥 杨栋梁 |
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| 作者单位： | 1. 中南大学化学化工学院制药工程系,长沙,410083
2. 苏州常熟市质量技术监督局,常熟,215500 |
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| 基金项目： | 教育部科研重点项目(No.03126)资助~~ |
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| 摘 要： | 以产紫杉醇类化合物的云南红豆杉内生真菌12.3.2为目标菌株,对其次生代谢物进行分离鉴定和抗菌活性研究。通过硅胶柱层析,共分离得到3个主要成分,红外光谱、质谱、核磁共振氢谱等光谱解析鉴定其分别为松柏烯、邻苯二甲酸二异辛酯、油酸乙酯。生物活性试验表明3种化合物对金黄色葡萄球菌、枯草杆菌、大肠杆菌、绿脓杆菌和白色链球菌这5种致病菌都有一定的抑制作用。其中松柏烯对金黄色葡球菌、枯草杆菌和白色念珠菌有较强的抑制作用。松柏烯是首次报道从植物内生真菌次生代谢物中分离得到,这为松柏烯的来源开辟了一条重要的途径,同时也为松柏烯的进一步开发利用奠定了基础。
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| 关 键 词： | 内生真菌 次生代谢物 抗菌活性 松柏烯 邻苯二甲酸二异辛酯 油酸乙酯 |
| 收稿时间： | 2008/10/14 0:00:00 |
Identification and antibacterial activity of secondary metabolites from Taxus endophytic fungus |
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| Shujuan Chen,Jiajia Liu,Hanxiang Gong and Dongliang Yang.Identification and antibacterial activity of secondary metabolites from Taxus endophytic fungus[J].Chinese Journal of Biotechnology,2009,25(3):368-374. |
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| Authors: | Shujuan Chen Jiajia Liu Hanxiang Gong and Dongliang Yang |
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| Institution: | Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;Suzhou Changshu?Bureau of Quality and Technique Supervision, Changshu 215500, China;Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China |
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| Abstract: | We isolated three secondary metabolites by silica gel column chromatography from endophytic fungus 12.3.2 that was isolated from Taxus yunnanensis and could produce taxanes. They were identified as cembrene (3,7,11-trimethyl-14-(1-methylethyl)-1,3,6,10-cyclotetradecatetraene), diisooctyl phthalate (1,2-benzenedicarboxylic acid diisooctylester) and ethyl oleate (9-octadecenoic acid-ethyl ester) by infrared spectra (IR), mass spectra (MS) and 1H-nuclear magnetic resonance (NMR). Their antibacterial activities... |
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| Keywords: | endophytic fungus secondary metabolite antibacterial activity cembrene 1 2-benzenedicarboxylic acid diisooctylester ethyl oleate |
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