In vitro regeneration of Trifolium glomeratum |
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Authors: | P Kaushal A Tiwari A K Roy D R Malaviya B Kumar |
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Institution: | (1) Crop Improvement Division, Indian Grassland and Fodder Research Institute, Jhansi, 284003, India |
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Abstract: | In vitro regeneration of Trifolium glomeratum, a leguminous forage species, was attempted through leaf, petiole, cotyledon, hypocotyl, collar and root explants and two
media combinations. Root and collar explants showed no callus induction. Medium with 0.05 mg dm−3 α-naphthaleneacetic acid (NAA) and 0.10 mg dm−3 N6-benzyladenine (BA) was more effective for hypocotyl explant whereas cotyledon and petiole explant were more responsive to
5.0 mg dm−3 NAA and 1.0 mg dm−3 BA. Friable, green calli obtained from petiole explant on this medium showed organogenetic potential. Modified root-inducing
medium having 0.21 mg dm−3 indole-3-acetic acid and 2.5 % sucrose was successful for root induction and plantlets were successfully transferred to field
after hardening and Rhizobium inoculation. |
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Keywords: | auxins clovers cytokinins tissue culture |
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