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Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from <Emphasis Type="Italic">Aegilops kotschyi</Emphasis> into Wheat |
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| Authors: | Shailender Kumar Verma Satish Kumar Imran Sheikh Prachi Sharma Priyanka Mathpal Sachin Malik Priyanka Kundu Anjali Awasthi Sundip Kumar Ramasare Prasad Harcharan Singh Dhaliwal |
| Institution: | 1.Department of Biotechnology,Indian Institute of Technology Roorkee,Roorkee,India;2.School of Life Sciences,Central University of Himachal Pradesh,Dharamshala,India;3.Akal College of Agriculture,Eternal University,Sirmour,India;4.Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities,G B Pant University of Agriculture and Technology,Pantnagar,India |
| Abstract: | Biofortification of bread wheat by the transfer of useful variability of high grain Fe and Zn from Aegilops kotschyi through induced homoeologous pairing is the most feasible approach to alleviate micronutrient malnutrition worldwide. Deficiency of chromosome 5B in interspecific hybrids allows homoeologous pairing and recombination of chromosomes of wheat with those of the related species. The interspecific hybrid plants without 5B chromosome showed much higher chromosome pairing than did the plants with 5B. The F1 plants without 5B chromosome were selected and repeatedly backcrossed with wheat cultivar PBW343. The chromosome number of BC2F1 plants ranged from 43 to 60 with several univalents and multivalents. Molecular markers and GISH analysis confirmed the introgression of U/S chromosomes of Ae. kotschyi and their fragments in wheat. The BC2F2 plants showed up to 125 % increase in Fe and 158 % increase in Zn compared to PBW343 with Lr24 and Yr36. Induced homoeologous pairing in the absence of 5B was found to be an effective approach for transfer of useful variability for enhanced grain Fe and Zn content for biofortification of wheat for high grain micronutrient content. |
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