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Interferon gamma gene +874A/T polymorphism and intracellular interferon gamma expression in pulmonary tuberculosis |
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| Authors: | S Prabhu Anand M Harishankar P Selvaraj |
| Institution: | 1. Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, PR China;2. Department of Tuberculosis, Third Hospital of Zhenjiang City, Zhenjiang 212005, PR China;3. Department of Breast, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, Nanjing 210004, PR China;1. Department of Internal Medicine 2, Friedrich-Alexander-University Erlangen, Germany;2. Department of Internal Medicine 1, Friedrich-Alexander-University Erlangen, Germany;1. Department of Emergency Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China;2. Department of Intensive Care Unit, The First Affiliated Hospital of Henan University of TCM, Zhengzhou, PR China |
| Abstract: | We investigated whether IFN-γ gene +874(A/T) polymorphism influences intracellular interferon gamma expression in T-cell subsets of normal healthy subjects (NHS) and pulmonary tuberculosis patients (PTB). Peripheral blood mononuclear cells were stimulated with live Mycobacterium tuberculosis (MTB) and the intracellular IFN-γ expression was studied using flow cytometry. Genotyping of IFN-γ gene +874(A/T) was done using allele specific polymerase chain reaction. Significantly increased IFN-γ expressing CD3+CD4+ and CD3+CD8+ T cells were observed in NHS with AA genotype compared to TT genotype in unstimulated (p = 0.0308 and p = 0.0157) and MTB stimulated (p = 0.0494 and p = 0.0287) cultures and this difference was not observed in PTB patients. The present study suggests that the variant genotypes of IFN-γ (+874) may be associated with altered expression of IFN-γ at the intracellular level and play an immunoregulatory role at the site of M. tuberculosis infection. |
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