条斑紫菜多糖PY-D2对4种人肿瘤细胞株生长的影响

目的:研究条斑紫菜多糖体外抗肿瘤的生物学活性。方法:应用生化技术分离和纯化条斑紫菜多糖,获得条斑紫菜多糖的2个组分,分别为PY-D1和PY-D2;在体外培养条件下分别用不同浓度的条斑紫菜多糖PY-D2处理4种人肿瘤细胞,通过MTT法观察条斑紫菜多糖PY-D2对4种人肿瘤细胞生长的影响;采用流式细胞仪检测肿瘤细胞的细胞周期变化。结果:条斑紫菜多糖PY-D2诱导HO-8910、MCF-7、K562和7721肿瘤细胞72h后,对其生长有明显的抑制作用,呈剂量依赖效应,500mg/LPY-D2的抑制率分别为21.2%、23.6%、19.8%和21%(P<0.001)。流式细胞仪检测表明PY-D2可以阻滞肿瘤细胞的细胞周期于G0/G1期或G2/M期。结论:条斑紫菜多糖PY-D2具有抑制肿瘤细胞HO-8910、MCF-7、K562和7721生长的作用,其有关的分子生物学作用机理值得进一步研究。

Effect of Porphyra yezoensis Polysaccharide PY-D2 on the Growth of Four Human Tumor Cell Lines

Objective:To observe the anti-tumor effects of Porphyra yezoensis polysaccharide.Methods:By extracting and purifing of polysaccharide from P.yezoensis,two components,PY-D1 and PY-D2,were isolated with ion-exchange chromatography DEAE-52.In vitro,the growth of four different human tumor cell lines were detected by MTT and flow cytometric analysis after induced by P.yezoensis polysaccharide PY-D2 for 72 h.Results:PY-D2 could significantly inhibit the growth of human tumor cells HO-8910,MCF-7,K562 and 7721.The inhibitory rates were 21.2%,23.6%,19.8% and 21% separately after cells were induced by 500 mg/L PY-D2 induced(P<0.001).The result of flow cytometry indicated that PY-D2 showed the antitumor effect of PY-D2 was relative to the blocking of G0/G1 or G2/M period cells.Conclusion:P.yezoensis polysaccharide PY-D2 has showed cytotoxicity effect on the tumor cells and may be a potential adjuvant to cancer therapy.