| 用聚合酶链反应-指纹图法分析HLA-DRB基因型 |
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| 引用本文： | 刘晓荣,王申五,李丹,于燕.用聚合酶链反应-指纹图法分析HLA-DRB基因型[J].中国生物化学与分子生物学报,1992,8(6):646-649. |
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| 作者姓名： | 刘晓荣 王申五 李丹 于燕 |
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| 作者单位： | 北京医科大学血液病研究所，北京医科大学血液病研究所，北京医科大学血液病研究所，北京医科大学血液病研究所 北京 100034，北京 100034，北京 100034，北京 100034 |
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| 摘 要： | 将不同个体的DNA经聚合酶链反应(PCR)扩增其HLA-DRB基因的高度多态区域,扩增产物经12％的聚丙烯酰胺凝胶电泳,照像后分析不同个体的指纹图型。PCR-指纹图法(PCR-Fingerprinting)为一种简便,快速的基因分析方法,可加速无关骨髓供者的筛选,它在器官移植及法医的个体识别中有一定实用价值。
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| 关 键 词： | PCR-指纹图 DRB基因配型 |
| 收稿时间： | 1992-12-20 |
HLA-DRB Gene Typing with PCR-Fingerprinting |
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| Liu,Xiao-rong Wang,Shen-wu Li,Dan Yu,Yan.HLA-DRB Gene Typing with PCR-Fingerprinting[J].Chinese Journal of Biochemistry and Molecular Biology,1992,8(6):646-649. |
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| Authors: | Liu Xiao-rong Wang Shen-wu Li Dan Yu Yan |
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| Institution: | (Beijing Medical University,Instite of Hematology,Beijing 100034 |
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| Abstract: | A simple and rapid method for HLA-DRB gene typing is discussed in this paper.After isolating DNAs from recipient and donors, the PCR with DRB primers were carried out.The partial products of patient and donors can be mixed for DNA crossmatching.The products were, then, analysed on 12% polyacrylamide gel elect-rophoresis.The fingerprinting of seven unrelated individuals were showed in different pattern.In four cases of patient-donor pairs, the two pairs gave matched pattern and the other pairs gave mismatched one.The results could be compared with HLA serologic typing, MLC and PCR-SSO DQA typing.This method would be useful to screen unrelated donor for BMT. |
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| Keywords: | PCR-Fingerprinting DRB gene typing |
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