Partial Purification and Characterization of the Vacuolar H+-ATPase of Mammalian Synaptic Vesicles |
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Authors: | Erik Floor Phillip S Leventhal Susan F Schaeffer |
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Institution: | Department of Anatomy, University of Wisconsin Medical School, Madison. |
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Abstract: | Several major proteins of synaptic vesicles from rat or cow brain sediment as a large complex on sucrose density gradients when solubilized in nonionic detergents. A vacuolar H(+)-ATPase identified by sensitivity to bafilomycin A1 appears to be associated with this oligomeric protein complex. Two subunits of this complex, synaptic vesicle proteins S and U, correspond to the 57-kDa (B) and 39-kDa accessory (Ac39) subunits, respectively, of bovine chromaffin granule vacuolar H(+)-ATPase as shown by Western immunoblot analysis. The five subunits of the oligomeric complex constitute approximately 20% of the total protein of rat brain synaptic vesicles. Taken together, these results strongly suggest that the abundant, multisubunit complex partially purified from brain synaptic vesicles by density gradient centrifugation is a vacuolar H(+)-ATPase. Bafilomycin A1 completely blocks proton pumping in rat brain synaptic vesicles as measured by 14C]methylamine uptake and also blocks catecholamine accumulation measured by 3H]dopamine uptake. Moreover, ATPase activity, 14C]methylamine uptake, and 3H]dopamine uptake are inhibited by bafilomycin A1 at similar I50 values of approximately 1.7 nmol/mg of protein. These findings indicate that the vacuolar H(+)-ATPase is essential for proton pumping as well as catecholamine uptake by mammalian synaptic vesicles. |
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Keywords: | Synaptic vesicles ATPase Dopamine uptake Proton pump Brain |
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