The Bacillus subtilis DnaD protein: a putative link between DNA remodeling and initiation of DNA replication |
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Authors: | Turner Ian J Scott David J Allen Stephanie Roberts Clive J Soultanas Panos |
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Institution: | Laboratory of Biophysics and Surface Analysis (LBSA), School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK. |
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Abstract: | The Bacillus subtilis DnaD protein is an essential protein and a component of the oriC and PriA primosomal cascades, which are responsible for loading the main replicative ring helicase DnaC onto DNA. We present evidence that DnaD also has a global DNA architectural activity, assembling into large nucleoprotein complexes on a plasmid and counteracting plasmid compaction in a manner analogous to that recently seen for the histone-like Escherichia coli HU proteins. This DNA-remodeling role may be an essential function for initiation of DNA replication in the Gram +ve B. subtilis, thus highlighting DnaD as the link between bacterial nucleoid reorganization and initiation of DNA replication. |
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Keywords: | DNA replication Atomic force microscopy DnaD Nucleoid |
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