Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli |
| |
Authors: | W Knoerzer H P Binder K Schneider P Gruss J E McCarthy W Risau |
| |
Institution: | PROGEN Biotechnik GmbH, Heidelberg, F.R.G. |
| |
Abstract: | Synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) were assembled and cloned using established Escherichia coli expression plasmids. Transformed E. coli cells were able to synthesize either a fusion protein, comprising the first seven amino acids of beta-galactosidase, a linker fragment and bovine FGF, or genomic human bFGF. The two growth factors were purified from E. coli lysates by cation exchange and heparin-Sepharose affinity chromatography. The purified recombinant proteins were biologically active as monitored by their mitogenic activity for bovine aortic endothelial cells and their angiogenic capacity in the rabbit cornea. |
| |
Keywords: | |
|
|