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Expression of recombinant human interleukin 6 ( rhIL6) in Saccharomyces cerevisiae by the modified phosphoglycerate kinase and chelatin promoter |
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| Authors: | K.-S. Chung H.-S. Kang K.-W. Kim I. Choi K.-H. Pyun H.-S. Yoo |
| Affiliation: | 1. Cell Cycle & Signal Research Unit, Korea Research Institute of Bioscience & Biotechnology, P.O.Box 115, Yusong Taejon, 305–606, Korea 2. Immune Cell Signal Transduction Research Unit, Korea Research Institute of Bioscience & Biotechnology, P.O.Box 115, Yusong Taejon, 305–606, Korea 3. Dept. of Molecular Biology, Pusan NationalUniversity, Pusan, 600–738, Korea |
| Abstract: | Using the modified yeast expression vectors that contained phosphoglycerate kinase or chelatin promoter with the adenine (A) base at –3 position from the ATG coding sequences and the leu-2 gene, recombinant human inter-leukin-6 ( rhIL-6) was produced as a b-galactosidase ( lacZ) fusion protein in Saccharomyces cerevisiae. Expression level of the IL-6-lacZ was 12 times higher than that from the unmodified wild type plasmids and IL-6 activity was 9–9.8 x 10 5 unit/mg yeast total protein. Thus these modified yeast vectors are useful for high expression of foreign genes in yeast |
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