| 家蝇胚胎细胞系的建立及其生物学特性 |
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| 引用本文： | 贺莉芳,万启惠,刘晖,张曦,阎玉涛. 家蝇胚胎细胞系的建立及其生物学特性[J]. 昆虫知识, 2009, 46(5): 788-790 |
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| 作者姓名： | 贺莉芳 万启惠 刘晖 张曦 阎玉涛 |
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| 作者单位： | |
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| 基金项目： | 贵州省科学技术厅基金，遵义市重大科技项目培育与科技人才培养计划项目，贵州省省长基金，珠海市科技局基金 |
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| 摘 要： | 昆虫细胞系在许多领域得到广泛的应用。以家蝇Musca domestica L.卵(胚胎)为材料,用含20%胎牛血清的M3培养基培养,原代培养35d左右形成单层,每7d传代1次,连续传代60代次。细胞群体倍增时间为44h,染色体2n=12,可在液氮及4℃冰箱中保存复苏,命名为MDEC-07114。
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| 关 键 词： | 家蝇 胚胎 细胞系 生物学特性 |
Establishment of cell line of Musca domestica embryo |
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| HE Li-Fang,WAN Qi-Hui,LIU Hui,ZHANG Xi,YAN Yu-Tao. Establishment of cell line of Musca domestica embryo[J]. Entomological Knowledge, 2009, 46(5): 788-790 |
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| Authors: | HE Li-Fang WAN Qi-Hui LIU Hui ZHANG Xi YAN Yu-Tao |
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| Affiliation: | ( Departement of Parasitology, Zunyi Medical College, Zunyi 563003, China) |
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| Abstract: | Insect cell lines have become useful tools in many research fields. Embryos of Musca domestica L. Wereused as experimental materials. The cells were cultured in M3 medium containing 20% fetal bovine serum. Afterabout 35-day primary culture, the cells could be passaged one time every 7 days, and were stably passaged for 60-generation period. The time of the cell population doublings was 44 h. The chromosomes are 2n = 12. The cells fromthis cell line could be stored at liquid nitrogen or 4℃ refrigerator, and resuscitated at room temperature. It was namedMDEC-07114. |
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| Keywords: | Musca domestica embryo cell line biological characteristics |
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