Purification and properties of pea cotyledon and embryo diamine oxidase |
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Authors: | S.K. Srivastava V. Prakash |
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Affiliation: | Biochemistry Department, Baroda University, Baroda, India |
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Abstract: | Diamine oxidase was purified separately from cotyledon and embryo of pea seedlings germinated for 6 days. The Km of the cotyledon enzyme for putrescine was 1.6 × 10?4M while that for the embryo enzyme was 9 × 10?5M. On heating for 15 min at 70° the embryo enzyme retained about 90% activity whereas the cotyledon enzyme retained only 20% activity. The electrophoretic mobility of the cotyledon enzyme was ca twice that of the enzyme from embryo. |
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Keywords: | pea diamine oxidase purification kinetics. |
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