Genetic diversity analysis among and within populations of Pogostemon cablin from China with ISSR and SRAP markers |
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| Authors: | You-Gen Wu Qiao-Sheng Guo Ji-Chan He You-Fen Lin Li-Juan Luo Guo-Dao Liu |
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| Institution: | 1. Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, PR China;2. Key Laboratory of Tropical Horticulture Plant Resources & Genetic Improvement Ministry of Education, College of Horticulture and Landscape, Hainan University, Haikou 570228, PR China;3. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratoy of Tropical Crops Germplasm Resources Utilization, Ministry of Agriculture, Danzhou 571737, PR China |
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| Abstract: | The diversity and genetic relationship among and within 16 populations of Pogostemon cablin from China were analyzed using ISSR and SRAP markers. High percentage of polymorphism observed by these two markers indicated a high level of genetic diversity existed among P. cablin populations. The percentage of polymorphic bands revealed by these two markers showed genetic variations were much higher between populations of P. cablin than within. The UPGMA clustering results using ISSR, SRAP or ISSR + SRAP combination showed most accessions from the same or adjacent regions were classified together. However, cluster results based on the absolute contents of patchouli alcohol and pogostone did not keep in good accordance with their geographic distributions, which revealed that the forming of patchouli alcohol and pogostone were relevant to the cultivation conditions except for genetic factors and environmental conditions. Finally, an appropriate strategy for conserving the patchouli germplasm was proposed. |
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| Keywords: | Pogostemon cablin (Blanco) Benth Genetic diversity ISSR SRAP Patchouli alcohol Pogostone |
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