Purification and characterization of caprine kidney uricase, possessing novel kinetic and thermodynamic properties |
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Authors: | MI Rajoka Khalil-ur- Rehman Tehmina Tabish MA Zia |
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Institution: | (1) National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan;(2) Department of Chemistry, University of Agriculture, Faisalabad, Pakistan |
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Abstract: | Purified uricase from a caprine kidney, possessed K
m
and V
max values of 1.1 mg ml−1 and 3512 IU (mg protein)−1 for uric acid hydrolysis, respectively. The optimum temperature and pH for catalytic activity were 40 °C and 8.5, respectively.
The activation energy for formation of ES complex was 13.6 kJ mol−1. Enthalpy (ΔH*), entropy of activation (ΔS*) and Gibbs free energy demand of uricase inactivation were 62.8 kJ mol−1, −102 J mol−1 K−1 and 104.3 kJ mol−1, respectively. Gibbs free enrgy demand for substrate binding and transition state stabilization were also determined which
were comparable with those for themostable enzymes. |
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Keywords: | Caprine uricase enthalpy entropy kinetics and thermodynamics temperature inactivation |
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