Structural requirements for rapid inactivation and voltage dependence in splice variants of lobster Shaker potassium channels |
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Authors: | French L B Singh A Luik R Harris-Warrick R M |
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Institution: | Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, USA. lfrench@allegheny.edu |
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Abstract: | We studied the properties of currents generated in Xenopus oocytes by nine splice variants of the spiny lobster Shaker gene. These isoforms differ in their amino termini and in the P-loop region of the pore. Both the voltage dependence and kinetic properties of the currents varied significantly, depending on which amino terminus was present. A cluster of net positive charges at the N-terminus was not necessary for rapid inactivation: negatively charged N-termini also inactivated rapidly. There was no obvious correlation between N-terminus length and inactivation rate. These N-terminal effects were additive with a separate set of voltage and kinetic properties controlled by the two alternative P-loop exons. |
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