Dansylcadaverine specific staining for transamidating enzymes. |
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| Authors: | L Lorand G E Siefring Y S Tong J Bruner-Lorand A J Gray |
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| Institution: | Department of Biochemistry and Molecular Biology, Northwestern University, Evanston, Illinois 60201, USA |
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| Abstract: | Versatile fluorescent staining methodologies, based on the incorporation of dansylcadaverineN-(5-aminopentyl)-5-dimethylamino-l-naphthalenesulfonamide] into N,N-dimethylcasein, are described for the detection of transamidating enzymes of the endo-γ-glutamine:ε-lysine transferase type. Activity staining was employed for comparing the electrophoretic behaviors of such transamidating enzymes derived from human and guinea pig tissues. Two enzymatically active forms of guinea pig liver transglutaminase were found. |
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| Keywords: | To whom reprint requests should be addressed |
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