盐度对暗纹东方鲀生长、非特异性免疫和抗氧化酶活力的影响

实验比较不同盐度处理对暗纹东方鲀（Takifugu obscurus）的生长、非特异性免疫和抗氧化酶活力的影响。实验设置6、12、18、24和30 5个盐度处理组，淡水组设为对照组。分别在实验的第1、第5、第10、第15、第20、第30、第40和第50天进行取样测定，并进行溶菌酶、碱性磷酸酶、超氧化物歧化酶和过氧化氢酶活力测定。当盐度18时，鱼的生长受影响较小，而在盐度为24和30的高盐度组中鱼体生长受到较大抑制，并且死亡率随着盐度的增加而增高。经盐度处理后，溶菌酶和碱性磷酸酶活力先逐渐增加，在达到最高峰后又逐渐降低，在24和30的高盐度组中，最终酶活力显著低于对照组水平。超氧化物歧化酶和过氧化氢酶的活力也呈现出先增加后降低的趋势，最终保持与对照组相似的水平。结果表明，高盐度（24和30）对暗纹东方鲀的生长、非特异性免疫和抗氧化酶活力均有显著的影响。  

EFFECTS OF SALINITY ON GROWTH,ACTIVITY OF NON-SPECIFIC IMMUNE AND ANTIOXIDANT ENZYMES IN OBSCURE PUFFER TAKIFUGU OBSCURES

Effects of salinity on the growth, the non-specific immunity and the antioxidant enzyme activities in Obscure puffer Takifugu obscures were investigated. The salinity of aquaculture water were maintained at 6, 12, 18, 24 and 30 in four experimental groups, and group of fresh water was used as control. We collected the samples on day 1, 5, 10, 15, 20, 30, 40 and 50 during the experiment, and we examined the activities of lysozyme, alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) in the samples. The salinity did not show significant impact on the growth when the salinity of culture water was below 18. However, the growth was inhibited when treated with salinity of 24 and 30, and the death rate was also elevated with the increase in salinity. The activities of both lysozyme and AKP was first enhanced by the increased salinity, but the activities in water with higher salinities (24 and 30) were significantly lower than that of in control groups. The activities of SOD and CAT also increased after treatment, then gradually decreased and the final level was not significantly different from control groups. The results showed that high salinity of 24 and 30 significantly affected the growth, non-specific immunity and the activities of antioxidant systems in obscure puffer Takifugu obscures.