| 地衣芽孢杆菌固态发酵麻疯树饼粕产蛋白酶及其酶学性质 |
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| 引用本文： | 吴远根,詹深山,吴洁,徐晓宝,杜亚菲,郭云兰,王超,邱树毅.地衣芽孢杆菌固态发酵麻疯树饼粕产蛋白酶及其酶学性质[J].微生物学通报,2010,37(2):0256-0262. |
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| 作者姓名： | 吴远根 詹深山 吴洁 徐晓宝 杜亚菲 郭云兰 王超 邱树毅 |
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| 作者单位： | 1. 贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳550003;上海交通大学环境科学与工程学院,上海200240
2. 贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳,550003 |
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| 基金项目： | 教育部“春晖”计划(No. Z2007-1-52017); 贵州省科学技术基金(黔科合J字[2007]2035号); 国家大学生创新性实验计划(No. 2008017) |
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| 摘 要： | 利用地衣芽孢杆菌作为出发菌株,以提油后的麻疯树饼粕作为培养基,采用固态发酵方式生产蛋白酶。控制培养基湿度为125%,添加10%的乳糖和5%的蛋白胨,30°C条件下发酵3d,蛋白酶产量达到最大值(7465U/g)。酶学性质研究表明,蛋白酶最适作用pH为5?6,最适催化温度为55°C,最大催化速度Vmax为0.0324μmol/(s·mg),Km值为0.0531mmol/L。有机溶剂对酶活力有明显促进作用,10%(V/V)甲醇和5%(V/V)乙醇可以使酶活力分别提高13.55%和70.9%。Mg2+可以使蛋白酶活力提高42.54%,而Hg2+却使酶彻底失活。
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| 关 键 词： | 麻疯树饼粕 地衣芽孢杆菌 蛋白酶 固态发酵 酶学性质 |
Production and Characterization of Protease from Bacillus licheniformis in Solid-state Fermentation Using Deoiled Jatro-pha curcas Seed Cake as Substrate |
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| WU Yuan-Gen,ZHAN Shen-Shan,WU Jie,XU Xiao-Bao,DU Ya-Fei,GUO Yun-Lan,WANG Chao and QIU Shu-Yi.Production and Characterization of Protease from Bacillus licheniformis in Solid-state Fermentation Using Deoiled Jatro-pha curcas Seed Cake as Substrate[J].Microbiology,2010,37(2):0256-0262. |
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| Authors: | WU Yuan-Gen ZHAN Shen-Shan WU Jie XU Xiao-Bao DU Ya-Fei GUO Yun-Lan WANG Chao and QIU Shu-Yi |
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| Institution: | 1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China; 2. School of Environmental Science & Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China;1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China;1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China;1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China;1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China;1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China;1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China |
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| Abstract: | |
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| Keywords: | Jatropha curcas seed cake (JCSC) Bacillus licheniformis Solidstate fermentation (SSF) Protease Protease characterization |
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