The chloroplast cytochrome b
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f complex can exist in monomeric and dimeric states |
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| Authors: | R K Chain R Malkin |
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| Institution: | (1) Department of Plant Biology, University of California, 111 Genetics and Plant Biology Building, 94720 Berkeley, CA, USA |
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| Abstract: | The cytochrome b
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f complex isolated from spinach chloroplast membranes can be resolved into two forms, a monomeric and a dimeric form, by centrifugation on sucrose gradients. The conversion of the dimeric form of the complex into the monomeric form could be prevented by cross-linking with the homobifunctional reagent, dithiobis(succinimidylpropionate) but not by cross-linking with disuccinimidyltartrate or glutaraldehyde. SDS-PAGE analyses of the monomeric and dimeric forms of the cytochrome complex showed the presence of specific cross-linked products in each respective form of the complex. For example, the monomeric form contained a cross-linked product of cytochrome f, cytochrome b
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f and subunit IV while the dimeric form contained a cross-linked dimer of cytochrome b
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f. The presence of the former in the isolated cytochrome b
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f complex prepared by the method of Hurt and Hauska (Eur J Biochem 117: 591–599, 1981) indicates the presence of the monomer in his preparation.Abbreviations DBMIB
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
- DSP
dithiobis(succinimidylpropionate)
- DST
disuccinimidyltartrate |
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| Keywords: | cytochrome b
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f complex plastoquinol-plastocyanin oxidoreductase cytochrome f cytochrome b
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f cross-linking |
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