Distribution of glutathione peroxidases glutathione reductase in rat brain mitochondria |
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Authors: | Enrico Panfili Gabriella Sandri Lars Ernster |
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Institution: | Instituto de Investigaciones Biomédicas del CSIC, Madrid, Spain. |
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Abstract: | The distribution of glutathione reductase (GR), glutathione peroxidase (GPx) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) in isolated rat brain mitochondria was investigated. using a fractionation procedure for the separation of inner and outer membranes, contact sites between the two membranes and a soluble fraction mainly originating from the mitochondrial matrix. The data indicate that GR and GPx are concentrated in the soluble fraction, with a minor portion of the two enzymes being associated with the contact sites. PHGPx is localized largely in the inner membrane. The possible functional significance of these findings is discussed. |
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Keywords: | Glutathione reductase Glutathione peroxidase Phospholipid hydroperoxide glutathione peroxidase Mitochondria Contact site Rat brain |
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