Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

Authors:	Shunji Hitotsubashi Yoshio Fujii Keinosuke Okamoto

Affiliation:	Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima 770, Japan; Institute of Pharmacognosy, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima 770, Japan

Abstract:	Abstract The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of ¹²⁵I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with ¹²⁵I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

Keywords:	Escherichia coli Heat-stable enterotoxin Receptor Membrane protein