白腹锦鸡和红腹锦鸡的遗传分化 |
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作者姓名: | 向余劲攻 杨岚 张亚平 |
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作者单位: | 1.中国科学院昆明动物研究所细胞与分子进化开放实验室,昆明 650223
2.中国科学院昆明动物研究所,昆明 650223
1.Laboratory of Cellular and Molecular Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650023,China
2.Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650023,China |
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基金项目: | 国家自然科学基金,云南省应用基础研究基金资助项目 |
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摘 要: | 白腹锦鸡Chrysolophus amherstiae (Leadbeater)和红腹锦鸡Chrysolophus pictus (L.)是两个种还是两个亚种尚有争议。测定了白腹锦鸡C.amherstiae两个个体部分细胞色素b基因序列,得到完全相同的861bp的序列。与红腹锦鸡C.pictus的同源序列相比,在21个位点上出现变异,应用木村资生的双参数法算出两者的遗传距离为2.5%。根据鸡形目细胞色素b基因核苷酸替换的速率约为每百万年0.5%~ 0.7%,推断出两种锦鸡的分化时间至少为1.7百万年。从而在分子水平上支持白腹锦鸡(C.amherstiae)和红腹锦鸡(C.pictus)是两个独立种。
Abstract:It was disputed that Chrysolophus amherstiae (Leadbeater) and Chrysolophus pictus (L.) were two species or two subspecies.DNA sequences spanning 861 nucleotide bases of the mitochondrial cytochrome-b gene were reported in two C.amherstiae.21 sites were different compared with C.pictus's homologous sequences. Genetic distance between C.amherstiae and was 2.5% based on the Kimura's two parameter's methods.The divergence time between C.amherstiae and C.pictus was at least 1.7My based on the substitution rate of nucleotide acid of cytothrome-b gene in Galliformes.We suggested that Chrysolophus amherstiae and Chrysolophus pictus are two distinct species.
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关 键 词: | 白腹锦鸡 红腹锦鸡 细胞色素b基因 Key words Chrysolophus amherstiae 种 |
修稿时间: | 2000-03-21 |
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