The binding of 5s ribosomal ribonucleic acid to ribosomal subunits |
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| Authors: | D G Comb N Sarkar |
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| Affiliation: | 1. State Key Laboratory of Remote Sensing Science, Jointly Sponsored by Aerospace Information Research Institute, Chinese Academy of Sciences and Beijing Normal University, Beijing 100101, China;2. School of Atmospheric Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Zhuhai 519082, Guangdong, China;3. CNR IBE, Institute of Bioeconomy, Via Caproni 8, Firenze, Italy;4. Department of Geography, University of Zurich, 8057 Zurich, Switzerland;5. Department of Environmental Systems Science, Institute of Agricultural Sciences, ETH Zurich, Universitätstrasse 2, CH-8092 Zurich, Switzerland;6. Global Change Research Institute, Czech Academy of Sciences, Bělidla 4a, 603 00 Brno, Czech Republic;7. Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China;8. Ministry of Education Key Laboratory for Geographical Environment Evolution, Nanjing 210023, China;1. Key Laboratory of Watershed Geographic Sciences, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;2. Ocean College, Zhejiang University, Zhoushan 316021, China;3. College of Urban and Environmental Sciences, Northwest University, Xi’an 710127, China;1. Mato Grosso State University (UNEMAT), Cáceres, MT, Brazil;2. São Paulo State University (UNESP), Botucatu, SP, Brazil;3. Rural University of Amazonia (UFRA), Belém, PA, Brazil;4. University of Padova (UNIPD), Padova, PD, Italy |
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| Abstract: | ![]()
The 75 s ribosomes of the aquatic fungus, Blastocladiella emersonii dissociate at 10−4 m-Mg2+ into 63 s and 45 s subunits. Only the 63 s subunit contains 5 s rRNA, about one molecule per subunit being present. When these subunits are treated with EDTA, the 63 s particle is unfolded to a 47 s particle without extensive release of protein but with the release of 5 s rRNA. Using 32P-labeled 5 s rRNA, we have demonstrated that 5 s rRNA will exchange with the 5 s rRNA on the 63 s subunit. Binding studies demonstrate that 2 molecules of 5 s rRNA will bind to ribosomal subunits or to the isolated 47 s large subunit. Binding to one of the two sites is displaced by transfer RNA. |
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