Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH |
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Authors: | Christoph A Gehring Robyn M McConchie Michael A Venis Roger W Parish |
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Institution: | (1) Department of Botany, La Trobe University, Bundoora, Victoria, 3083, Australia, AU;(2) Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, UK, GB |
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Abstract: | Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a putative auxin-binding
domain of the auxin-binding protein ABP1, induce stomatal opening and decrease guard-cell pHi, as monitored with the acetomethoxy ester of the ratiometric pH indicator Snarf-1. Similar activity was shown by a monoclonal
antibody against the same domain. The C-terminal dodecapeptide, Pz152–163 of maize ABP1 (ABPzm1) induced guard-cell alkalinization
and closed stomata, as did Fab fragments of a monoclonal antibody (MAC 256) recognising the C-terminal region of ABPzm1. By
implicating, for the first time, an auxin-binding protein in mediation of an auxin-dependent physiological response, these
findings strongly support an auxin-receptor role for ABP1.
Received: 23 December 1997 / Accepted: 16 January 1998 |
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Keywords: | : Alkalinization Auxin receptor Cytoplasmic pH Guard cell Paphiopedilum Stomatal opening |
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