Gold immunochromatographic assay for simultaneous detection of carbofuran and triazophos in water samples |
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Authors: | Yi-Rong Guo |
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Institution: | Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China |
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Abstract: | Using a simple test for rapid identification and quantification of pesticide multiresidues in food and environmental samples is a long-cherished approach for practical monitoring purposes. Here two gold-based lateral-flow strips (strip A and strip B) were investigated for simultaneous detection of carbofuran and triazophos. For the strip A format, a bispecific monoclonal antibody (BsMcAb) against both carbofuran and triazophos was employed to prepare the immunogold probe. For the strip B format, anti-carbofuran monoclonal antibody (McAb) and anti-triazophos McAb separately labeled with colloidal gold were combined as detector reagents. By comparison of visual results from pesticide standard tests between the two formats, the strip B assay manifested higher sensitivities for both pesticides. Analysis of spiked water samples by the preferable strip indicated that the detection limits for carbofuran and triazophos were 32 and 4 μg/L, respectively. The strength of the portable one-step strip assay was in the simultaneous screening for two pesticides within a short time (8-10 min) without any equipment. |
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Keywords: | Carbofuran Triazophos Colloidal gold Lateral-flow strip assay Bispecific monoclonal antibody |
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