Cloning, Expression, and Homology Modeling of GroEL Protein from Leptospira interrogans Serovar Autumnalis Strain N2 |
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| Authors: | Kalimuthusamy Natarajaseenivasan Santhanam Shanmughapriya Sridhar Velineni Sergey C Artiushin John F Timoney Medical Microbiology Laboratory |
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| Institution: | Kalimuthusamy Natarajaseenivasan1,2,Santhanam Shanmughapriya1,Sridhar Velineni2,Sergey C. Artiushin2,and John F. Timoney2 1Medical Microbiology Laboratory,Department of Microbiology,School of Life Sciences,Bharathidasan University,Tiruchirappalli 620024,India,2Gluck Equine Research Center,University of Kentucky,Lexington,KY 40546,USA |
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| Abstract: | Leptospirosis is an infectious bacterial disease caused by Leptospira species. In this study, we cloned and se- quenced the gene encoding the immunodominant protein GroEL from L. interrogans serovar Autumnalis strain N2, which was isolated from the urine of a patient during an outbreak of leptospirosis in Chennai, India. This groEL gene encodes a protein of 60 kDa with a high degree of homology (99% similarity) to those of other leptospiral serovars. Recombinant GroEL was overexpressed in Escherichia coli. ... |
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| Keywords: | GroEL expression homology modeling leptospirosis serodiagnosis |
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