Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence. |
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| Authors: | C Elkins C E Thomas H S Seifert P F Sparling |
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| Affiliation: | Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599. |
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| Abstract: | Piliated Neisseria gonorrhoeae are known to be transformed less readily if transforming DNA competes with DNA containing the 10-bp sequence GCCGTCTGAA. It has been postulated that the 10-bp sequence is a recognition sequence which is required for efficient DNA uptake. We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid. |
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