Nuclear genome characterization of the carrageenophyteAgardhiella subulata (Rhodophyta) |
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Authors: | Donald F Kapraun Julie A Dutcher Juan Lopez-Bautista |
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Institution: | (1) Center for Marine Science Research, University of North Carolina-Wilmington, 7205 Wrightsville Avenue, Wilmington, North Carolina, 28403, U.S.A. |
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Abstract: | DNA reassociation kinetics were used to determine nuclear genome organization and complexity inAgardhiella subulata (Gigartinales, Rhodophyta). Results indicate the presence of three second-order components corresponding to fast (22%), intermediate
(68%) and slow (10%) fractions. Thus, the genome consists of 90% repetitive sequences. Microspectrophotoometry with the DNA-localizing
fluorochrome DAPI was used to confirm ploidy level differences in the gametophytic and tetrasporophytic phases. Results indicate
that meiosis occurs during tetrasporogenesis. Comparison of mean nuclear DNA (If) values to chicken erythrocytes (RBC) resulted in an estimate of 0.9 pg/2C genome forAgardhiella. Karyological studies using aceto-orcein revealed a chromosome complement of 2N = 44 in carposporangia and the presence of 22 bivalents during diakinesis of tetraspore mother cells. |
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Keywords: | Agardhiella carrageenophyte cytophotometry Gigartinales nuclear genome reassociation kinetics |
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