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On-line monitoring of enzymes in downstream processing by flow injection analysis (FIA) |
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| Authors: | Achim Recktenwald Karl-Heinz Kroner Maria-Regina Kula |
| Institution: | GBF, Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300 Braunschweig, FRG |
| Abstract: | Flow injection analysis (FIA) has been employed to automate enzyme assays for formate dehydrogenase (FDH) and l-leucine dehydrogenase (l-LeuDH). Coupled to a special sampling device the FIA assays were used to monitor on-line downstream processes, e.g. disintegration of microbial cells and cross-flow filtration of cell homogenates. |
| Keywords: | On-line monitoring flow injection analysis automated enzyme assay formate dehydrogenase l-leucine dehydrogenase disintegration diafiltration cross-flow filtration |
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