维生素E对中华鳖幼鳖生长、肝脏维生素E以及血清皮质醇含量的影响

为探讨维生素E(VE)对中华鳖(Pelodiscus sinensis)幼鳖的生长、肝脏VE和血清皮质醇的影响，通过特定生长率、高压液相色谱法和放免法，我们测定了中华鳖幼鳖的生长、肝脏VE和血清皮质醇含量。发现VE添加量为1000和5000mg／kg的两组，能明显降低中华鳖幼鳖的生长。维生素E添加量为500、1000和5000mg／kg的三组，肝脏维生素E含量明显高于对照组，VE添加量在0—1000mg／kg的范围时，肝脏VE的含量随着饲料中VE含量的增加呈指数式增加，并且在VE添加量为5000kg／kg的一组基本达到饱和。维生素E添加量为0和50mg／kg的2组，其血清皮质醇的平均值明显高于维生素E添加量为250、500、1000和5000mg／kg的4组的平均值。上述结果表明：高剂量的VE降低了中华鳖幼鳖的生长和血清皮质醇的含量；在一定剂量范围内，肝脏VE随着饲料中VE含量的增加而升高。

Effects of vitamin E on growth, liver vitamin E and serum cortisol level in juvenile soft-shelled turtles (Pelodiscus sinensis)

To investigate the effects of vitamin E on growth, liver vitamin E and the serum cortisol level of juvenile soft-shelled turtles, we estimated the growth in juvenile soft-shelled turtles by specific growth rate, analyzed liver vitamin E with a high pressure liquid chromatogram (HPLC) and determined serum cortisol content using the radiate immune method. Growth in juvenile soft-shelled turtles was depressed in two groups that received vitamin E supplementation at 1 000 and 5 000 mg/kg of diet. Levels of liver vitamin E in turtles that received vitamin E supplementation at 500, 1 000 and 5 000 mg/kg of diet were significantly higher than in the control group. The level of liver vitamin E increased exponentially with increasing dietary vitamin E within the range 0-1 000 mg/kg of diet, reaching saturation when dietary vitamin E reached 5 000 mg/kg of diet. The mean serum cortisol content in two groups that received vitamin E supplementation at 0 and 50 mg/kg of diet was significantly higher than that in four groups supplemented at 250, 500, 1 000 and 5 000 mg/kg of diet. These results suggest that high doses of vitamin E reduce growth rates and serum cortisol levels in juvenile soft-shelled turtles, and that liver vitamin E increases with increasing dietary vitamin E within certain limits[Acta Zoologica Sinica 49(1):40-44,2003].