Towards unambiguous assignment of methyl-containing residues by double and triple sensitivity-enhanced HCCmHm-TOCSY experiments |
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Authors: | Peter Würtz Maarit Hellman Helena Tossavainen Perttu Permi |
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Institution: | (1) Program in Structural Biology and Biophysics, Institute of Biotechnology/NMR Laboratory, University of Helsinki, P.O. Box 65, FI-00014 Helsinki, Finland |
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Abstract: | Chemical shift assignment of methyl-containing residues is essential in protein NMR spectroscopy, as these residues are abundant
in protein interiors and provide the vast majority of long-range NOE connectivities for structure determination. These residues
also constitute an integral part of hydrophobic cavities, the surroundings for many enzymatic reactions. Here we present a
powerful strategy for the assignment of methyl-containing residues in a uniformly 13C/15N double labeled protein sample. The approach is based on novel four-dimensional HCCmHm-TOCSY experiments, two of them utilizing
gradient selection and sensitivity enhancement in all three indirectly detected dimensions. Regardless of the number of dimensions,
the proposed experiments can be executed using only one transient per FID, providing outstanding resolution and sensitivity.
A complete assignment of the 51 methyl-containing residues in the 16 kDa Mus musculus coactosin was accomplished using a four-dimensional HCCmHm-TOCSY spectrum recorded in 16 hours. |
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Keywords: | assignment coactosin coherence transfer HCCH-TOCSY methyl groups NMR spectroscopy proteins |
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